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Evaluating mRNA expression of tax , B chain of PDGF and PDGF‐β receptors as well as HTLV‐I proviral load in ATL patients and healthy carriers
Author(s) -
Atabati Hadi,
Esmaeili SeyedAlireza,
Allahyari Abolghasem,
Shirdel Abbas,
Rahimi Hossein,
Rezaee Seyed Abdolrahim,
MomtaziBorojeni Amir A.,
Rafatpanah Houshang
Publication year - 2021
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.26510
Subject(s) - platelet derived growth factor receptor , peripheral blood mononuclear cell , platelet derived growth factor , receptor , leukemia , t cell leukemia , growth factor , biology , messenger rna , virology , immunology , microbiology and biotechnology , cancer research , medicine , gene , genetics , in vitro
Adult T‐cell leukemia (ATL) is a life‐threatening malignant neoplasm of CD4 + T cells resulted from human T‐cell leukemia virus type I (HTLV‐I). Tax1 protein of HTLV‐I can induce malignant proliferation of T‐cells by modulating the expression of growth factors such as platelet‐derived growth factor (PDGF). Here, we aimed to investigate the proviral load (PVL) of HTLV‐I in ATL and also to evaluate the mRNA expression of B chain of PDGF and PDGF‐β receptors in ATL patients and HTLV‐I‐infected healthy carriers. To this end, peripheral blood mononuclear cells (PBMCs) were isolated by using Ficoll‐Histophaque density centrifugation. The mean of HTLV‐I PVL in ATL patients (42,759 ± 15,737 copies/10 4 cells [95% CI, 9557‐75962]) was significantly ( p = .01) higher than that in healthy carriers (650 ± 107 copies/10 4 cells [95% CI, 422‐879], respectively. The HTLV‐I PVL in ATL patients exhibited a significant correlation with PBMC count ( R = .495, p = .001). The mRNA expression of Tax, B chain of PDGF, and PDGF‐β receptor genes was significantly higher in healthy carriers than in patients with ATL. In conclusion, the expression of the canonical PDGFβ and its receptor, and their correlation with Tax expression cannot be a suitable indicator and/or prognostic factor for progression of ATL in HTLV‐I carriers.