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Molecular typing of conjunctivitis‐causing adenoviruses in Hanoi, Vietnam from 2017 to 2019 and complete genome analysis of the most prevalent type (HAdV‐8)
Author(s) -
Nguyen Thi Thu Huyen,
Le Tuan Anh,
Nguyen Viet Ha,
Nguyen Thi Uyen,
Nguyen Phuong Thao,
Tran Thi Thuy Anh,
Nguyen Quang Hung,
Hoang Anh Tuan,
Hoang My Hanh,
Le Tho Son,
Nguyen Van Sang
Publication year - 2020
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.25844
Subject(s) - virology , polymerase chain reaction , biology , sanger sequencing , genome , typing , phylogenetic tree , gene , mastadenovirus , dna sequencing , recombinant dna , genetics , adenoviridae
Adenoviral conjunctivitis is a common epidemic worldwide. In Vietnam, up to 80,000 patients are infected with adenoviral conjunctivitis annually. However, there are few investigations on the pathogenic adenoviruses that cause conjunctivitis. In total, 120 eye‐swab samples were collected from patients with viral conjunctivitis symptoms in Hanoi, Vietnam from 2017 to 2019. Human adenoviruse (HAdV) was detected in 67 samples (55.83%) using polymerase chain reaction amplification of at least one of three HAdV‐specific marker genes (hexon, penton, and fiber). Of the 67 HAdV samples, 46 samples could be analyzed by all three marker genes. DNA sequence analysis and phylogenetic tree building based on the three marker genes from the 46 HAdV samples revealed five different HAdV types associated with conjunctivitis in Hanoi, including HAdV‐3 (4.3%), HAdV‐4 (2.2%), HAdV‐8 (89.1%), HAdV‐37 (2.2%), and a potential recombinant type between types HAdV‐8 and HAdV‐3 (2.2%). This showed that HAdV‐8 was the most common type identified in Hanoi. Complete genome analysis of HAdV‐8 isolated from a Vietnamese patient (VN2017) using Sanger sequencing revealed 34 unique nucleotide changes, indicating that the adenovirus continuously accumulates new mutations. Hence, continuous surveillance of HAdV‐8 changes in Vietnam is necessary in the future.