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The construction of a new oncolytic herpes simplex virus expressing murine interleukin‐15 with gene‐editing technology
Author(s) -
Cai Linkang,
Hu Han,
Duan Haixiao,
Li Yuying,
Zou Zongxing,
Luo Kailun,
Zhang Ziyi,
Yang Junhan,
Jin Jing,
Chen Ying,
Ke Zonghuang,
Fang Zongyao,
Liu Qiong,
Hong Xiaoqian,
Hu Sheng,
Liu Binlei
Publication year - 2020
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.25691
Subject(s) - virology , oncolytic virus , herpes simplex virus , gene , virus , biology , genetics
The treatment of tumors with oncolytic viruses is an important cancer immunotherapy strategy. Interleukin‐15 (IL‐15) can enhance the antitumor effect of natural killer cells and T cells. An oncolytic herpes simplex type II virus (oHSV2‐mIL‐15CherryFP) expressing mouse IL‐15 was constructed using the CRISPR/Cas9 system, and its antitumor activity in vitro and in vivo was evaluated. In vitro, the mouse interleukin‐15 (mIL‐15) present in the culture supernatant expressed by oHSV2‐mIL‐15CherryFP was able to enhance the killing of CT26‐GFP tumor cells by T cells. In addition, the intratumoral injection of oHSV2‐mIL‐15CherryFP inhibited tumor growth in the CT26‐iRFP and BGC823‐iRFP model. These results indicate that the use of oncolytic herpes simplex virus expressing IL‐15 may be a potential therapeutic strategy in tumor immunotherapy.