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IL6 and FAS/FASL gene polymorphisms may be associated with disease progression in HIV‐1‐positive ethnically mixed patients
Author(s) -
Loureiro dos Reis Marília M.,
Queiroz Maria A. F.,
Silva Bosco C. M.,
Silva Duarte Alberto J.,
Casseb Jorge,
Arganaraz Gustavo A.,
Vallinoto Antonio C. R.,
Argañaraz Enrique R.
Publication year - 2020
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.25651
Subject(s) - genotype , genotyping , fas ligand , biology , allele , immune system , taqman , immunology , polymorphism (computer science) , apoptosis , virology , gene , genetics , polymerase chain reaction , programmed cell death
The progression of AIDS depends on the complex host and virus interactions. The most important disease progression hallmarks are immune activation and apoptosis. In this study, we address the prevalence of polymorphisms related to proinflammatory and apoptotic genes, such as IFNG (+874T/A), TNF (308G/A), IL6 (−174G/C), IL8 (−251A/T), FAS (−670A/G), and FASL (−124A/G) in 160 ethnically mixed HIV‐1‐infected patients from multicentre cohorts with different clinical outcomes (13 elite controllers [EC], 66 slow long‐term non‐progressors [LTNPs], and 81 progressors [P]). The genotyping was accomplished by TaqMan‐qPCR. Among all the polymorphisms analyzed in the cytokines, the IL6 −174G/C polymorphism showed a higher frequency of GG genotype in the LTNP and LTNP+EC groups as compared to the P group. Moreover, there was a significantly higher frequency of the G allele in the LTNP and LTNP+EC groups as compared to the P group. On the other hand, the levels of CD4 + T lymphocytes were higher among individuals showing the AA and AG genotypes for the FASL −124A/G polymorphism as compared to the GG genotype. Furthermore, the AG and AA genotypes were more frequent, as compared to the GG genotype, in individuals showing a lower viral load. In contrast, for the FAS −670A/G polymorphism, a significantly higher viral load was observed in individuals with the AG genotype as compared to the GG genotype. In conclusion, we found three genetic allelic variants of the IL6 −174G/C, FASL −124A/G, and FAS −670A/G polymorphisms that were related to disease progression and immunological and virological markers in cohorts of HIV‐1‐positive ethnically mixed patients.