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Development of an HIV‐1 integrase genotyping assay for HIV‐1 subtype AE
Author(s) -
Liu Yang,
Margot Nicolas,
Kitrinos Kathryn M.,
Callebaut Christian
Publication year - 2019
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.25376
Subject(s) - genotyping , integrase , virology , reverse transcriptase , biology , polymerase chain reaction , lentivirus , virus , group specific antigen , human immunodeficiency virus (hiv) , population , reverse transcription polymerase chain reaction , drug resistance , gene , microbiology and biotechnology , genotype , viral disease , genetics , medicine , messenger rna , environmental health
An human immunodeficiency virus (HIV)‐1 integrase (IN) genotyping assay was developed to evaluate clinical samples from patients infected with HIV‐1 subtype AE, a subtype highly prevalent in Asia. The HIV‐1 IN gene was amplified from plasma‐derived HIV‐1 viral RNA via reverse transcription polymerase chain reaction followed by population sequencing. Assay sensitivity was also evaluated using serially diluted plasma samples. The genotyping assay successfully amplified the IN gene from patient plasma samples with HIV‐1 RNA as low as 500 copies/mL. This assay is suitable for IN genotyping to identify IN drug resistance mutations in HIV‐1 patients harboring subtype AE virus.