Premium
Assessing hepatitis B immunity using dried blood spot samples from HIV+ individuals
Author(s) -
Flores Geane Lopes,
Cruz Helena Medina,
Miguel Juliana Custódio,
Potsch Denise Vigo,
Pilotto José Henrique,
LewisXimenez Lia Laura,
Lampe Elisabeth,
Villar Livia Melo
Publication year - 2018
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.25275
Subject(s) - dried blood spot , immunoassay , context (archaeology) , dried blood , virology , antibody , titer , medicine , hepatitis b virus , human immunodeficiency virus (hiv) , hepatitis b , immunology , virus , vaccination , antibody titer , immunity , viral disease , biology , immune system , chemistry , paleontology , genetics , chromatography
This study aims to evaluate the utility of an optimized enzyme immunoassay (EIA) to detect and quantify antibodies against hepatitis B surface antibody (anti‐HBs) in dried blood spots (DBSs) within the context of human immunodeciency virus (HIV) status. Serum and DBS samples were obtained from 56 HIV+ and 99 HIV− patients and subjected to EIA for the detection of anti‐HBs, where sample volume and cut off value were modified for DBS testing. Sensitivities of anti‐HBs detection in DBS were 79.8% and 76.8% in HIV− and HIV+ subjects, respectively. Concordant results for anti‐HBs in serum and DBS presented high mean CD8 cell counts, HIV viral load and optical density (OD) values of anti‐HBs. Anti‐HBs titers were significantly higher in serum, whether or not anti‐HBs titers were detected in DBS. It was possible to detect anti‐HBs in DBS as low as 17.4 and 27.3 IU/mL among HIV+ and HIV− subjects, respectively. In conclusion, DBS can be used to detect and quantify anti‐HBs in HIV‐infected individuals, which could increase access to diagnosis and vaccination.