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Chromosomally integrated human herpesvirus 6 in the Japanese population
Author(s) -
Miura Hiroki,
Kawamura Yoshiki,
Hattori Fumihiko,
Kozawa Kei,
Ihira Masaru,
Ohye Tamae,
Kurahashi Hiroki,
Yoshikawa Tetsushi
Publication year - 2018
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.25244
Subject(s) - cohort , incidence (geometry) , virology , population , serology , cord blood , nested polymerase chain reaction , biology , polymerase chain reaction , immunology , antibody , medicine , genetics , gene , physics , environmental health , optics
The objectives of the work are to elucidate the incidence and virological findings of chromosomally integrated human herpesvirus 6 (ciHHV‐6) in Japanese population and to analyze an association between ciHHV‐6 and the clinical manifestation of exanthema subitum (ES). Real‐time polymerase chain reaction was performed to determine HHV‐6 DNA loads in 2347 cord blood samples from healthy neonates (cohort A), febrile children less than 5 years old (cohort B), and hematopoietic cell transplant recipients (cohort C). CiHHV‐6 was confirmed by detection of high copy numbers of viral DNA in somatic cells. The integration site was determined by fluorescent in situ hybridization analysis. In the ciHHV‐6 subjects of cohorts A and B, HHV‐6 antibody titers were measured, the history of ES was obtained, and the incidence of ES was compared with non–ciHHV‐6 children without primary HHV‐6B infection in the cohort B. CiHHV‐6 was detected in 14 (0.60%) of the 2347 samples: A (6/1006, 0.60%), B (6/790, 0.76%), and C (2/551, 0.36%). The integration sites were on chromosome 22q in seven cases, Yp in two cases, and 17q and Xp in one case. No past history of ES was observed in 11 of the 12 subjects. Nine children with ciHHV‐6 underwent serological analysis and were found to be positive for HHV‐6 IgG antibodies. Incidence of ES was statistically higher in the control subjects than the ciHHV‐6 subjects ( P = 0.0039). In Japan, the frequency of ciHHV‐6 was 0.60%. A high incidence of ciHHV‐6A, specifically in chromosome 22, is a characteristic finding among the Japanese. CiHHV‐6 may interfere with the clinical symptoms of primary HHV‐6B infection.