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Molecular typing and whole genome next generation sequencing of human adenovirus 8 strains recovered from four 2012 outbreaks of keratoconjunctivitis in New York State
Author(s) -
Lamson BS Daryl M.,
Kajon Adriana E.,
Shudt Matthew,
Quinn Monica,
Newman Alexandra,
Whitehouse Joan,
Greenko Jane,
Adams Eleanor,
George Kirsten
Publication year - 2018
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.25223
Subject(s) - outbreak , typing , keratoconjunctivitis , virology , neonatal intensive care unit , intensive care , biology , restriction enzyme , medicine , microbiology and biotechnology , pediatrics , genetics , intensive care medicine , gene
Ocular infections caused by human adenovirus (HAdV) are highly contagious. The most severe are usually caused by members of species HAdV‐D (types HAdV8, 19, 37, 53, 54, and 56) and can manifest as epidemic keratoconjunctivitis (EKC), often resulting in prolonged impairment of vision. During the early months of 2012, EKC outbreaks occurred in neonatal intensive care units (NICUs) in 3 hospitals in New York State (New York and Suffolk Counties). A total of 32 neonates were affected. For 14 of them, HAdV8 was laboratory‐confirmed as the causative agent. Nine healthcare workers were also affected with 3 laboratory‐confirmed, HAdV‐positive EKC. A fourth EKC outbreak was documented among patients attending a private ophthalmology practice in Ulster County involving a total of 35 cases. Epidemiological linkage between the neonatal intensive care unit outbreaks was demonstrated by molecular typing of virus isolates with restriction enzyme analysis and next generation whole genome sequencing. The strain isolated from the ophthalmology clinic was easily distinguishable from the others by restriction enzyme analysis.