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Indeterminate genotypes of hepatitis C virus by the Abbott RealTi m e HCV Genotype II assay in Morocco. About eight cases resolved by a sequencing method
Author(s) -
Mesbahi Zineb,
Kabbaj Hakima,
Malki Houcine,
Bouihat Najat,
Qrafli Mounia,
Belefquih Bouchra,
Marcil Sarrah,
Challine Dominique,
Pawlotsky JeanMichel,
Bouvier Magali,
Seffar Myriam
Publication year - 2018
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.25191
Subject(s) - genotype , genotyping , virology , indeterminate , hepatitis c virus , biology , virus , flaviviridae , genetics , gene , mathematics , pure mathematics
The early detection and genotyping of the hepatitis C virus (HCV) are important in the management of this infection. The genotype is the major factor influencing treatment decisions. That's why it is necessary to use fast and accurate methods in its determination. This study reports, over a period of 3 years (from May 2012 to July 2015), the percentage of indeterminate genotypes by the Abbott RealTi m e HCV Genotype II test and their results using a sequencing technique. Of 309 samples of 309 patients tested, 11 were indeterminate (4.4%). There were three cases of cross‐reactivity (1b/4 in one case, 2/5 in two cases) and a possible co‐infection 1 + 4. Among those indeterminate genotypes, cross‐reactivities and co‐infections, ten samples were tested by sequencing. The results were for four of them a 1d subtype, five were a 2i subtype and one was a 2l subtype. These results support the thesis of complementarity between the two methods: genotyping for the detection of mixed reactions and sequencing for resolving indeterminate cases by genotyping.