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Characterization of unusual DS‐1‐like G3P[8] rotavirus strains in children with diarrhea in Japan
Author(s) -
Komoto Satoshi,
Ide Tomihiko,
Negoro Manami,
Tanaka Takaaki,
Asada Kazutoyo,
Umemoto Masakazu,
Kuroki Haruo,
Ito Hiroaki,
Tanaka Shigeki,
Ito Mitsue,
Fukuda Saori,
Suga Shigeru,
Kamiya Hajime,
Nakano Takashi,
Taniguchi Koki
Publication year - 2018
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.25016
Subject(s) - rotavirus , genotype , phylogenetic tree , biology , diarrhea , virology , genome , genetics , gene , phylogenetics , virus , medicine
The emergence and rapid spread of novel DS‐1‐like intergenogroup reassortant rotaviruses having the equine‐like G3 genotype (DS‐1‐like G3P[8] strains) have been recently reported from several countries. During rotavirus surveillance in Japan in 2015–2016, three DS‐1‐like G3P[8] strains were identified from children with severe diarrhea. In the present study, we sequenced and characterized the full genomes of these three strains. On full‐genomic analysis, all three strains showed a unique genotype constellation including both genogroup 1 and 2 genes: G3‐P[8]‐I2‐R2‐C2‐M2‐A2‐N2‐T2‐E2‐H2. Phylogenetic analysis revealed that each of the 11 genes of the three strains was closely related to that of Japanese DS‐1‐like G1P[8] and/or Japanese equine‐like G3P[4] human strains. Thus, the three study strains were suggested to be reassortants that acquired the G3‐VP7 gene from equine G3 rotaviruses on the genetic background of DS‐1‐like G1P[8] strains. Our observations will provide important insights into the evolutionary dynamics of emerging DS‐1‐like G3P[8] strains.