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A concordance study of the Altona RealStar Varicella‐Zoster virus real‐time quantitative PCR and in‐house conventional qualitative PCR
Author(s) -
Grandjean Lapierre Simon,
Boissinot Karel,
Renaud Christian
Publication year - 2018
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24957
Subject(s) - varicella zoster virus , concordance , real time polymerase chain reaction , medicine , virology , kappa , virus , cerebrospinal fluid , biology , genetics , philosophy , gene , linguistics
This study compared the Altona RealStar™ VZV Kit 1.0 real time quantitative VZV PCR with in‐house qualitative conventional VZV PCR on cerebrospinal fluid, mucocutaneous, and other uncommon clinical specimens. Overall, positive and negative agreement percentages were respectively 97.9% (95%CI: 93.8‐99.6), 100.0% (95%CI: 93.1‐100.0), and 96.3% (95%CI: 89.4‐99.2) while Cohen's kappa statistic value was 0.96 (95%CI: 0.91‐1.00). RealStar™ VZV quantitative PCR assay reported average quantitative viral loads of 4.4 × 10 5 and 1.1 × 10 7 copies/mL in cerebrospinal fluid and cutaneous specimens, respectively ( P < 0.01). RealStar™ VZV PCR assay showed excellent agreement with in house conventional assay for various clinical specimens.