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Identification of group B rotavirus as an etiological agent in the gastroenteritis outbreak in Maharashtra, India
Author(s) -
Joshi Madhuri S.,
Ganorkar Nital N.,
Ranshing Sujata S.,
Basu Atanu,
Chavan Nutan A.,
Gopalkrishna Varanasi
Publication year - 2017
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24901
Subject(s) - rotavirus , outbreak , virology , etiology , identification (biology) , acute gastroenteritis , reoviridae , geography , biology , medicine , virus , ecology , psychiatry
Acute gastroenteritis outbreak occurred at Pargaon, Maharashtra, India in 1789 cases with an attack rate of 32.5% between November to December 2015. The stool specimens ( n = 32) were investigated for different enteric viral agents using conventional methods. Transmission electron microscopy and RNA polyacrylamide gel electrophoresis respectively identified morphologically distinct rotavirus particles in 28% and RNA migration pattern of Group B Rotavirus (GBR) in 72% of the specimens. Reverse transcription polymerase chain reaction and nucleotide sequencing confirmed presence of GBR in 97% of the samples analyzed. The predominance of GBR infections and absence or insignificant presence of other agents confirmed GBR as an etiological agent of the gastroenteritis outbreak occurred in Maharashtra, India.