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Anal HPV 16 and 18 viral load: A comparison between HIV‐negative and ‐positive MSM and association with persistence
Author(s) -
Marra Elske,
King Audrey,
van Logchem Elske,
van der Weele Pascal,
Mooij Sofie H.,
Heijman Titia,
Meijer Chris J.L.M.,
Verhagen Dominique W.M.,
van der Sande Marianne A. B.,
Schim van der Loeff Maarten F.
Publication year - 2018
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24898
Subject(s) - viral load , persistence (discontinuity) , men who have sex with men , virology , logistic regression , titer , human immunodeficiency virus (hiv) , medicine , immunology , biology , virus , geotechnical engineering , syphilis , engineering
Does anal HPV viral load explain the difference in anal HPV persistence between HIV‐negative and ‐positive men who have sex with men (MSM)? MSM ≥18 years were recruited in Amsterdam, the Netherlands, in 2010‐2011. Anal self‐swabs were collected every 6 months and genotyped (SPF 10 ‐PCR‐DEIA‐LIPA 25 ‐system). HPV16 and HPV18 load was determined with a type specific quantitative (q)PCR, and compared between HIV‐negative and ‐positive men using ranksum test. Persistence was defined as ≥3 positive samples for the same HPV‐type. Determinants of persistent HPV16/18 infection and its association with HPV16/18 load were assessed with logistic regression. Of 777 recruited MSM, 54 and 22 HIV negative men were HPV16 and HPV18 positive at baseline, and 64 and 39 HIV‐positive MSM. The geometric mean titer (GMT) of HPV16 was 19.6 (95%CI 10.1‐38.0) and of HPV18 8.6 (95%CI 2.7‐27.5) DNA copies/human cell. HPV16 and HPV18 load did not differ significantly between HIV‐negative and ‐positive MSM ( P = 0.7; P = 0.8, respectively). In multivariable analyses HPV16 load was an independent determinant of HPV16 persistence (OR 1.8, 95%CI 1.3‐2.4). No difference in anal HPV viral load was found between HIV‐positive and HIV‐negative MSM. HPV 16/18 viral load is an independent determinant of type‐specific persistence.