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Detection of long term cellular immune response to Japanese encephalitis vaccination using IFN‐γ ELIspot assay
Author(s) -
Zia Amreen,
Singh Dharamveer,
Saxena Swati,
Umrao Jyoti,
Baluni Manjari,
Ghildiyal Sneha,
Fatima Tanzeem,
Shukla Mukti,
Agarwal Vikas,
Dhole Tapan N.
Publication year - 2017
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24893
Subject(s) - elispot , vaccination , immunology , immune system , virology , immunity , cellular immunity , medicine , encephalitis , japanese encephalitis vaccine , virus , japanese encephalitis , biology , t cell
Vaccine is the most effective preventive measure against Japanese Encephalitis infection. Role of IFN‐γ expressing T cells for JE virus clearance has been described as a part of cellular immunity. Vaccine induced immunity also involve the cellular immune response, therefore the study was aimed to observe induction and persistence of IFN‐γ expressing T cells by IFN‐γ ELISpot assay. The cell count increased significantly after 28 ( P < 0.0001) days post vaccination, and remained higher at all time points (day 28, day 180, day 360) when compared with prevaccination. This study will be helpful for designing future vaccination strategy and improving vaccine efficacy.