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Molecular surveillance of dengue in Minas Gerais provides insights on dengue virus 1 and 4 circulation in Brazil
Author(s) -
Dutra Karina Rocha,
Drumond Betânia Paiva,
de Rezende Izabela Maurício,
Nogueira Maurício Lacerda,
de Oliveira Lopes Débora,
Calzavara Silva Carlos Eduardo,
Siqueira Ferreira Jaqueline Maria,
dos Santos Luciana Lara
Publication year - 2017
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24729
Subject(s) - dengue fever , dengue virus , virology , arbovirus , genotype , capsid , outbreak , molecular epidemiology , biology , dengue vaccine , virus , gene , genetics
Dengue, caused by any of the four types of Dengue virus (DENV) isthe most important arbovirus in the world. In this study we performed a molecular surveillance of dengue during the greatest dengue outbreak that took place in Divinópolis, Minas Gerais state, Southeast Brazil, in 2013. Samples from 100 patients with clinical symptoms of dengue were studied and 26 were positive. The capsid/premembrane (CprM) and envelope gene sequences of some samples were amplified and sequenced. Molecular analyses demonstrated that two DENV‐1 lineages, belonging to genotype V were introduced and co‐circulated in Divinópolis. When compared to each other, those lineages presented high genetic diversity and showed unique amino acids substitutions in the envelope protein, including in domains I, II, and III. DENV‐4 strains from Divinópolis clustered within genotype IIb and the most recent common ancestor was probably introduced into the city three years before the 2013 epidemic. Here we demonstrated for the first time the circulation of DENV‐4 and the co‐circulation of two DENV‐1 lineages in Midwest region of Minas Gerais, Brazil. Moreover our analysis indicated the introduction of five DENV‐1 lineages, genotype V into Brazil, in different times. J. Med. Virol. 89:966–973, 2017 . © 2016 Wiley Periodicals, Inc.