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Genetic variability of respiratory syncytial virus A in hospitalized children in the last five consecutive winter seasons in Central Spain
Author(s) -
Calderón Ana,
Pozo Francisco,
Calvo Cristina,
GarcíaGarcía Mluz,
GonzálezEsguevillas Mónica,
Molinero Mar,
Casas Inmaculada
Publication year - 2017
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24703
Subject(s) - bronchiolitis , genotype , virus , respiratory system , virology , epidemiology , biology , respiratory tract infections , asthma , respiratory disease , lower respiratory tract infection , paramyxoviridae , medicine , pediatrics , gene , immunology , viral disease , genetics , lung
Human respiratory syncytial virus group A (RSV‐A) was detected in symptomatic hospital attended children in Central Spain for a continuous time period, September 2010 to April 2015. In order to accurately describe the epidemiology of this virus, the genetic diversity of the complete G gene and the clinical manifestations observed were jointly analyzed. Out of 3,011 respiratory specimens taken from 2,308 children, 640 were positive to RSV (21.3%) and 405 were RSV‐A (63.2%). Complete G gene sequences of 166 randomly selected RSV‐A virus identified NA1 and ON1 genotypes. In 2011–2012, ON1 emerged sporadically and become dominant in 2012–2013 with 38 cases (70%). In 2014–2015, all the 44 sequences contained the 72‐nt duplication (100%). Clinical diagnosis of children with ON1 genotype were bronchiolitis in 55 (62.5%), recurrent wheezing or asthma exacerbations in 22 (25%), laryngotracheobronchitis in 3 (3.4%), and upper respiratory tract infections in eight. Results showed replacement and substitution of circulating NA1 genotype with the new ON1 genotype. Nevertheless, at this stage, none of the RSV‐A genotypes identified have resulted in significant clinical differences. The amino acid composition of the complete G gene ON1 sequences demonstrated an accumulation of single changes not related with different clinical presentation. J. Med. Virol. 89:767–774, 2017 . © 2016 Wiley Periodicals, Inc.