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Predominant prevalence of human rotaviruses with the G1P[8] and G8P[8] genotypes with a short RNA profile in 2013 and 2014 in Sukhothai and Phetchaboon provinces, Thailand
Author(s) -
Guntapong Ratigorn,
Tacharoenmuang Ratana,
Singchai Phakapun,
Upachai Sompong,
Sutthiwarakom Karun,
Komoto Satoshi,
Tsuji Takao,
Tharmaphornpilas Piyanit,
Yoshikawa Tetsushi,
Sangkitporn Somchai,
Taniguchi Koki
Publication year - 2017
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24669
Subject(s) - rotavirus , virology , polyacrylamide gel electrophoresis , virus , acute gastroenteritis , genotype , rna , biology , gene , genetics , biochemistry , enzyme
Of 2,754 stool specimens collected from children with acute gastroenteritis during 2013–2014 in Sukhothai and Phetchaboon provinces, Thailand, 666 (24.2%) were positive for rotavirus A (RVA) in polyacrylamide gel electrophoresis (PAGE). The G and P types of all RVA‐positive specimens were determined by semi‐nested RT‐PCR. G1P[8] (56.5%) was most prevalent, followed by G2P[4] (22.1%). Unusual G8P[8] human RVAs (HuRVAs) were detected at a high frequency (20.0%). Interestingly, 171 of the 376 G1P[8] HuRVAs and all of the 133 G8P[8] HuRVAs showed a short RNA pattern in PAGE. Thus, it was shown that the properties of HuRVAs have been markedly unusual in recent years in Thailand. J. Med. Virol. 89:615–620, 2017 . © 2016 Wiley Periodicals, Inc.