Premium
High rate of missed HIV infections in individuals with indeterminate or negative HIV western blots based on current HIV testing algorithm in China
Author(s) -
Liu ManQing,
Zhu ZeRong,
Kong WenHua,
Tang Li,
Peng JinSong,
Wang Xia,
Xu Jun,
Schilling Robert F.,
Cai Thomas,
Zhou Wang
Publication year - 2016
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24490
Subject(s) - indeterminate , nat , virology , human immunodeficiency virus (hiv) , nucleic acid test , antibody , blot , medicine , immunoassay , western blot , algorithm , biology , immunology , covid-19 , disease , infectious disease (medical specialty) , gene , statistics , mathematics , pure mathematics , biochemistry , computer science
It remains unclear if China's current HIV antibody testing algorithm misses a substantial number of HIV infected individuals. Of 196 specimens with indeterminate or negative results on HIV western blot (WB) retrospectively examined by HIV‐1 nucleic acid test (NAT), 67.57% (75/111) of indeterminate WB samples, and 16.47% (14/85) of negative WB samples were identified as NAT positive. HIV‐1 loads in negative WB samples were significantly higher than those in indeterminate WB samples. Notably, 86.67% (13/15) of samples with negative WB and double positive immunoassay results were NAT positive. The rate of HIV‐1 infections missed by China's current HIV testing algorithm is unacceptably high. Thus, China should consider using NAT or integrating fourth generation ELISA into current only antibodies‐based HIV confirmation. J. Med. Virol. 88:1462–1466, 2016 . © 2016 Wiley Periodicals, Inc.