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Sequencing ebola and marburg viruses genomes using microarrays
Author(s) -
Hardick Justin,
Woelfel Roman,
Gardner Warren,
Ibrahim Sofi
Publication year - 2016
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24487
Subject(s) - ebolavirus , outbreak , virology , ebola virus , genome , dna microarray , marburg virus , biology , filoviridae , genetics , virus , viral disease , gene , gene expression , paramyxoviridae
Periodic outbreaks of Ebola and Marburg hemorrhagic fevers have occurred in Africa over the past four decades with case fatality rates reaching as high as 90%. The latest Ebola outbreak in West Africa in 2014 raised concerns that these infections can spread across continents and pose serious health risks. Early and accurate identification of the causative agents is necessary to contain outbreaks. In this report, we describe sequencing‐by‐hybridization (SBH) technique using high density microarrays to identify Ebola and Marburg viruses. The microarrays were designed to interrogate the sequences of entire viral genomes, and were evaluated with three species of Ebolavirus (Reston, Sudan, and Zaire), and three strains of Marburgvirus (Angola, Musoke, and Ravn). The results showed that the consensus sequences generated with four or more hybridizations had 92.1–98.9% accuracy over 95–99% of the genomes. Additionally, with SBH microarrays it was possible to distinguish between different strains of the Lake Victoria Marburgvirus . J. Med. Virol. 88:1303–1308, 2016 . © 2016 Wiley Periodicals, Inc.