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Multiplex‐PCR for the detection of viruses in the CSF of infants and young children
Author(s) -
Walls Tony,
McSweeney Alice,
Anderson Trevor,
Jennings Lance C.
Publication year - 2017
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24461
Subject(s) - multiplex , virology , enterovirus , multiplex polymerase chain reaction , viral meningitis , biology , population , virus , meningitis , medicine , polymerase chain reaction , bacterial meningitis , pediatrics , bioinformatics , gene , genetics , environmental health
We aimed to establish the utility of the Fast‐track diagnostics Viral meningitis multiplex PCR kit for the diagnosis of central nervous system infection in infants. The multiplex assay had reduced sensitivity for the detection of enterovirus, the predominant pathogen in young infants, when compared to our in‐house singleplex PCR. In our infant population, multiple singleplex PCR assays perform better than a multiplex assay for the detection of CSF viruses. J. Med. Virol. 89:559–561, 2017 . © 2016 Wiley Periodicals, Inc.