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Genetic diversity of HPV16 and HPV18 in Brazilian patients with invasive cervical cancer
Author(s) -
Vidal Joao Paulo C.B.,
Felix Shayany Pinto,
Chaves Cláudia B.P.,
Patury Patrícia,
Franco Vanessa F.,
de Morais Evaneide A.,
de Carvalho Neile A.,
Carvalho Aurenice C.L.,
Almeida Neto Olimpio F.,
Vieira Lina Maria T.M.,
Correa Flavia Miranda,
Martins Luís Felipe Leite,
Negrão Antonio,
de Almeida Liz Maria,
Moreira Miguel Angelo Martins
Publication year - 2016
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24458
Subject(s) - genotyping , lineage (genetic) , cervical cancer , biology , genetic diversity , genotype , phylogenetic tree , cancer , virology , cohort , genetics , medicine , gene , population , environmental health
Cervical cancer is the fourth most common cancer among women, and ∼70–80% of these cancers are associated with two human papillomavirus types: HPV16 and HPV18. Several studies have reported that intra‐type diversity is associated with the progression of infection to invasive cancer. Herein, we report the genetic diversity of HPV16 and HPV18 in a cohort of 594 Brazilian women with invasive cervical cancer and describe the prevalence of lineages and intra‐type diversity prior to the implementation of the public immunization program in Brazil. HPV detection and genotyping were performed using PCR, PGMY/GP primers, and DNA extracted from fresh tumors. The HPV16 (378 women) and HPV18 (80 women) lineages were identified by PCR and sequencing of the LCR and E6 fragments, followed by SNV comparison and phylogenetic analysis. In our cohort, was found a higher frequency of the lineage A (in 217 women), followed by lineage D (in 97 women) and lineages B and C (in 10 women each) for HPV16; and a higher frequency of lineage A (in 56 women) followed by lineage B (in 15 women) in HPV18. The genetic diversity of HPV16 indicated a recent expansion of specific variants or a selective advantage that is associated with invasive cancer; this pattern was not observed for HPV18. J. Med. Virol. 88:1279–1287, 2016 . © 2015 Wiley Periodicals, Inc.