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Epstein–Barr and cytomegalovirus DNA salivary shedding correlate with long‐term plasma HIV RNA detection in HIV‐infected men who have sex with men
Author(s) -
Scaggiante Renzo,
Andreis Samantha,
Basso Monica,
Franchin Elisa,
Franzetti Marzia,
Del Vecchio Claudia,
Torti Carlo,
Mengoli Carlo,
Cruciani Mario,
Sarmati Loredana,
Palù Giorgio,
Parisi Saverio Giuseppe
Publication year - 2016
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24441
Subject(s) - viral shedding , saliva , viral load , medicine , virology , immunology , men who have sex with men , virus , cytomegalovirus , human immunodeficiency virus (hiv) , gastroenterology , viral disease , herpesviridae , syphilis
The aim of the study was to evaluate cytomegalovirus (CMV) and Epstein–Barr virus (EBV) DNA salivary shedding in HIV‐positive men who have sex with men (MSM) and to determine whether viro‐immunological parameters and long‐term (24 months) plasma HIV RNA (pHIV) detection may predict herpesviruses replication. A total of 193 HIV‐positive MSM were consecutively recruited (mean CD4+ cell count 607 cells/mm 3 and mean nadir value 333 cells/mm 3 ); pHIV was analyzed for 24 months prior to saliva sampling: patients were categorized as successfully suppressed (SS) and not suppressed (NS). The EBV viral load was categorized as high viral load (HVL), intermediate (IVL), or low (LVL), CMV DNA as positive or negative. NS patients experienced both herpesviruses detectability more frequently respect to SS patients ( P = 0.034); conversely, no salivary shedding was more frequent in SS patients ( P = 0.014). HVL EBV was more frequent in NS patients than in SS subjects ( P = 0.038 for isolated EBV detection and P = 0.001 when CMV shedding was associated). NS subjects with HVL EBV had a median pHIV of 43,820 copies/ml, significantly higher respect to IVL and LVL patients ( P = 0.027 and P = 0.0005, respectively). CMV shedding was mostly associated to EBV shedding. NS patients showed a significantly higher frequency of saliva HVL EBV detection compared to SS patients; moreover, NS patients with HVL EBV had a higher pHIV respect to those with IVL and LVL shedding. Our results suggest that a successful pHIV suppression could reduce the burden of salivary EBV replication and likely the risk of herpesviruses‐related cancers. J. Med. Virol. 88:1211–1221, 2016 . © 2015 Wiley Periodicals, Inc.