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Differential effects of three echovirus strains on cell lysis and insulin secretion in beta cell derived lines
Author(s) -
Sarmiento Luis,
Medina Anya,
Aziz Kosrat,
Anagandula Mahesh,
CabreraRode Eduardo,
Fex Malin,
Frisk Gun,
Cilio Corrado M.
Publication year - 2016
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24438
Subject(s) - echovirus , beta cell , cell culture , biology , cytopathic effect , insulinoma , virology , insulin , cytolysis , enterovirus , cell , secretion , beta (programming language) , virus , islet , endocrinology , in vitro , biochemistry , cytotoxic t cell , genetics , computer science , programming language
In an earlier study, infection of human pancreatic islets with epidemic strains of echovirus (E4, E16, E30), with proven but differently ability to induce islet autoimmunity, resulted either in a severe damage (i.e., E16 and E30) or proceeded without visible changes in infected islets (i.e., E4). In this study, the ability of these strains to replicate in beta cells and the consequence of such an infection for beta cell lysis and beta cell function was studied in the pancreatic beta cell lines INS‐1, MIN6, and NIT‐1. The strains of E16 and E30 did replicate in INS1, MIN6, and NIT1 cells and resulted in a pronounced cytopathic effect within 3 days following infection. By contrast, E4 replicated in all examined insulinoma cells with no apparent cell destruction. The insulin release in response to high glucose stimulation was hampered in all infected cells ( P < 0.05) when no evidence of cytolysis was present; however, the adverse effect of E16 and E30 on insulin secretion appeared to be higher than that of the E4 strain. The differential effects of echovirus infection on cell lysis, and beta cell function in the rodent insulinoma INS1, MIN6, and NIT 1 cells reflect those previously obtained in primary human islets and support the notion that the insulin—producing beta cells can harbor a non‐cytopathic viral infection. J. Med. Virol. 88:971–978, 2016 . © 2015 Wiley Periodicals, Inc.