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Molecular epidemiology and surveillance of circulating rotavirus and adenovirus in Congolese children with gastroenteritis
Author(s) -
Mayindou Gontran,
Ngokana Berge,
Sidibé Anissa,
Moundélé Victoire,
KoukouikilaKoussounda Felix,
Christevy Vouvoungui Jeannhey,
Kwedi Nolna Sylvie,
Velavan Thirumalaisamy P.,
Ntoumi Francine
Publication year - 2016
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24382
Subject(s) - rotavirus , serotype , genotype , diarrhea , virology , epidemiology , molecular epidemiology , medicine , vomiting , pediatrics , biology , virus , gene , biochemistry
Infectious Diarrhea caused by rotavirus and adenovirus, is a leading cause of death in children in sub‐Sahara Africa but there is limited published data on the diverse rotavirus genotypes and adenovirus serotypes circulating in the Republic of Congo. In this study, we investigated the prevalence of severe diarrhea caused by rotavirus A (RVA) and Adenovirus serotype 40 and 41 in Congolese children hospitalized with severe gastroenteritis. Stool samples were collected from 655 Congolese children less than 60 months of age hospitalized with acute gastroenteritis between June 2012 and June 2013. Rotavirus and adenovirus antigens were tested using commercially available ELISA kits and the RVA G‐ and P‐ genotypes were identified by seminested multiplex RT‐PCR. Three hundred and four (46.4%) children were tested positive for RVA. Adenovirus infection was found in 5.5% of the 564 tested children. Rotavirus infection was frequently observed in children between 6–12 months (55.9%). The dry season months recorded increased RVA infection while no seasonality of adenovirus infection was demonstrated. The most common RVA genotypes were G1 (57.5%), G2 (6.4%), G1G2 mixture (15.5%), P[8] (58%), P[6] (13.2%), and P[8]P[6] mixture (26%). Additionally, the genotype G12P[6] was significantly associated with increased vomiting. This first study on Congolese children demonstrates a high prevalence and clinical significance of existing rotavirus genotypes. Adenovirus prevalence is similar to that of other Central African countries. This baseline epidemiology and molecular characterization study will contribute significantly to the RVA surveillance after vaccine implementation in the country. J. Med. Virol. 88:596–605, 2016 . © 2015 Wiley Periodicals, Inc.