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An infection of human adenovirus 31 affects the differentiation of preadipocytes into fat cells, its metabolic profile and fat accumulation
Author(s) -
BilLula Iwona,
KrzywonosZawadzka Anna,
Sawicki Grzegorz,
Woźniak Mieczysław
Publication year - 2016
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24362
Subject(s) - adipogenesis , adipose tissue , biology , lipid droplet , leptin , fatty acid synthase , intracellular , lipid metabolism , cell culture , fatty acid binding protein , peroxisome proliferator activated receptor , cellular differentiation , adipocyte , fibroblast , microbiology and biotechnology , endocrinology , biochemistry , gene , genetics , obesity
The primary issue undertaken in this study was to test the hypothesis that preadipocytes would have intrinsically elevated propensity to differentiate into mature adipocytes due to HAdV31 infection. To prove that, the metabolic and molecular mechanisms responsible for HAdV31‐induced adipogenesis were examined. 3T3L1 cells (mouse embryonic fibroblast, adipose like cell line) were used as a surrogate model to analyze an increased proliferation, differentiation, and maturation of preadipocytes infected with human adenovirus. An expression of E4orf1, C/EBP‐β, PPAR‐γ, GAPDH, aP2, LEP, and fatty acid synthase genes, intracellular lipid accumulation as well as cytokine release from the fat cells were assessed. Data showed that HAdV31 increased an expression of C/EBP‐β and PPAR‐γ genes leading to an enhanced differentiation of preadipocytes into fat cells. Besides, overexpression of GAPDH and fatty acid synthase, and decreased expression of leptin caused an increased accumulation of intracellular lipids. Secretion of TNF‐α and IL‐6 from HAdV31‐infected cells was strongly decreased, leading to unlimited virus replication. The results obtained from this study provided the evidences that HAdV31, likewise previously documented HAdV36, is a subsequent human adenovirus affecting the differentiation and lipid accumulation of 3T3L1 cells. J. Med. Virol. 88:400–407, 2016 . © 2015 Wiley Periodicals, Inc.