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Performance of rapid tests for discrimination between HIV‐1 and/or HIV‐2 infections
Author(s) -
GautheretDejean Agnès,
Bocobza Jonathan,
Brunet Sylvie,
Damond Florence,
Plantier JeanChristophe,
Barin Francis
Publication year - 2015
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24282
Subject(s) - human immunodeficiency virus (hiv) , virology , medicine , sida , viral load , viral disease , immunology
Major differences exist between HIV‐1 and HIV‐2 in terms of epidemiology, pathogenicity, sensitivity to antiretrovirals. Determining the type of HIV infecting a patient is essential for management. The aim of this study was to evaluate the ability of simple/rapid tests to differentiate between HIV‐1 and/or HIV‐2 infections. We analyzed 116 samples from patients infected with HIV‐1 (n = 61), HIV‐2 (n = 47), or HIV‐1+HIV‐2 (n = 8) at the chronic stage of infection. Each sample was tested with SD Bioline HIV‐1/2 3.0, ImmunoFlow HIV1–HIV2, ImmunoFlow HIV1–HIV2 (WB), Genie III HIV‐1/HIV‐2, ImmunoComb HIV1&2 BiSpot. HIV‐1, or HIV‐2 single infection was identified with a sensitivity ranging from 90% to 100%. The ability to detect dual infection was less sensitive (12.5–100%). SD Bioline HIV‐1/2 3.0, ImmunoFlow HIV1–HIV2, and Genie III were unable to detect HIV‐1 group O infection in one, one and two cases, respectively. The specificity of detection of HIV‐1, HIV‐2, or HIV‐1+HIV‐2 antibodies differed greatly (36–100%). ImmunoComb BiSpot had the highest sensitivity values (99–100% for HIV‐1, 98% for HIV‐2, and 75–87.5% for dual infection) and specificity values (94–100% for HIV‐1, 100% for HIV‐2, and 97–100% for dual infection). In conclusion, this study showed that no single rapid test had a perfect sensitivity/specificity ratio, particularly in the case of the double infections. J. Med. Virol. 87:2061–2066, 2015 . © 2015 Wiley Periodicals, Inc.