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HPV E6/E7 mRNA versus HPV DNA biomarker in cervical cancer screening of a group of Macedonian women
Author(s) -
Duvlis Sotirija,
PopovskaJankovic Katerina,
Arsova Zorica Sarafinovska,
Memeti Shaban,
Popeska Zaneta,
PlaseskaKaranfilska Dijana
Publication year - 2015
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24199
Subject(s) - cervical cancer , medicine , multiplex , colposcopy , oncology , cancer , biomarker , cytology , dysplasia , carcinogenesis , gynecology , biology , pathology , bioinformatics , genetics
Abstract High risk types of human papillomaviruses E6/E7 oncogenes and their association with tumor suppressor genes products are the key factors of cervical carcinogenesis. This study proposed them as specific markers for cervical dysplasia screening. The aim of the study is to compare the clinical and prognostic significance of HPV E6/E7 mRNA as an early biomarker versus HPV DNA detection and cytology in triage of woman for cervical cancer. The study group consists of 413 women: 258 NILM, 26 ASC‐US, 81 LSIL, 41 HSIL, and 7 unsatisfactory cytology. HPV4AACE screening, real‐time multiplex PCR and MY09/11 consensus PCR primers methods were used for the HPV DNA detection. The real‐time multiplex nucleic acid sequence‐based assay (NucliSENS EasyQ HPV assay) was used for HPV E6/E7 mRNA detection of the five most common high risk HPV types in cervical cancer (16, 18, 31, 33, and 45). The results show that HPV E6/E7 mRNA testing had a higher specificity 50% (95% CI 32–67) and positive predictive value (PPV) 62% (95% CI 46–76) for CIN2+ compared to HPV DNA testing that had specificity of 18% (95% CI 7–37) and PPV 52% (95% CI 39–76) respectively. The higher specificity and PPV of HPV E6/E7 mRNA testing are valuable in predicting insignificant HPV DNA infection among cases with borderline cytological finding. It can help in avoiding aggressive procedures (biopsies and over‐referral of transient HPV infections) as well as lowering patient's anxiety and follow up period. J. Med. Virol. 87:1578–1586, 2015 . © 2015 Wiley Periodicals, Inc.