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Enumeration of NKG2C + natural killer cells early following allogeneic stem cell transplant recipients does not allow prediction of the occurrence of cytomegalovirus DNAemia
Author(s) -
Giménez Estela,
Solano Carlos,
Amat Paula,
de la Cámara Rafael,
Nieto José,
López Javier,
GarciaNoblejas Ana,
Navarro David
Publication year - 2015
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24198
Subject(s) - cytomegalovirus , cd8 , human cytomegalovirus , virology , immunology , transplantation , flow cytometry , biology , medicine , virus , viral disease , herpesviridae , immune system
The role of Natural killer (NK) cells in the control of cytomegalovirus (CMV) infection in allogeneic stem cell transplant recipients has not been precisely characterized. The current study is aimed at investigating the potential role of NK cells expressing the activating receptor NKG2C in affording protection against the development of CMV DNAemia in patients exhibiting detectable CMV‐specific CD8 + T‐cell responses early following transplantation. A total of 61 nonconsecutive patients were included in the study. Peripheral levels of CD56 bright CD16 −/low and CD56 dim CD16 + NKG2C + NK cells and CMV pp65/IE‐1‐specific IFN‐γ‐producing CD8 + T‐cells were enumerated by flow cytometry at days +30 and +60 after transplant. Neither the absolute number of NKG2C + NK cells, nor that of CD56 bright CD16 −/low and CD56 dim CD16 + NKG2C + NK‐cell subsets at day 30 differed significantly between patients with or without subsequent CMV DNAemia. No significant correlation was found between levels of both NKG2C + NK‐cell populations and the peak CMV DNA load within subsequent episodes of CMV DNAemia. The data indicate that enumeration of NKG2C + NK cells early after transplant is unlikely to be helpful in identifying those patients at highest risk of developing CMV DNAemia. Moreover, the data do not support a direct implication of NKG2C + NK cells in preventing the development of CMV DNAemia. J. Med. Virol. 87:1601–1607, 2015 . © 2015 Wiley Periodicals, Inc.