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Clinical performance of multiplex high‐risk e6 mrna expression in comparison with hpv dna subtypes for the identification of women at risk of cervical cancer
Author(s) -
Ho ChihMing,
Pan KuiYou,
Chen YunYuan,
Huang ChiaYen,
Chen YuLi,
Chang ShwuFen
Publication year - 2015
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24194
Subject(s) - cervical intraepithelial neoplasia , cervical cancer , odds ratio , messenger rna , multiplex , polymerase chain reaction , confidence interval , medicine , cancer , real time polymerase chain reaction , biology , oncology , gastroenterology , gynecology , gene , bioinformatics , genetics
We compared multiplex E6 messenger ribonucleic acid (mRNA) tests using real‐time quantitative reverse transcriptase polymerase chain reactions (PCR) with human papillomavirus (HPV) DNA subtypes using a MY11/GP6+ PCR‐based reverse‐blot assay to identify cervical intraepithelial neoplasias of grade 2 or worse (CIN2+). In total, 684 women were studied, of whom 377 (55%) were diagnosed with CIN2+ histologically. The specificity of HPV mRNA to predict histological CIN2+ was higher than that of HPV DNA (81.3% vs. 44.2%). The odds ratios (ORs) to predict histological CIN2+ in women with positive for type 16, 18, 31, and 45 E6 mRNA or by HPV DNA detection were 7.1 (95% confidence interval [CI] 3.9–13.1) and 2.5 (95%CI 1.9–3.5), respectively, compared to those with negative for E6 mRNA or HPV DNA. The OR to predict histological CIN2+ in women with a cytological grade
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