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Molecular detection and characterization of BK and JC polyomaviruses in urine samples of renal transplant patients in Southern Brazil
Author(s) -
Comerlato Juliana,
Campos Fabrício Souza,
Oliveira Martha Trindade,
Cibulski Samuel Paulo,
Corrêa Lilian,
Kulmann Marcos Iuri Roos,
Arantes Thalita Souza,
Hentges Lucas Peretti,
Spilki Fernando Rosado,
Roehe Paulo Michel,
Franco Ana Cláudia
Publication year - 2015
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24086
Subject(s) - bk virus , renal transplant , virology , urine , polyomavirus infections , jc virus , medicine , kidney , kidney transplantation , virus , progressive multifocal leukoencephalopathy
The human polyomaviruses JC (JCPyV) and BK (BKPyV) are widespread in the human population. Following the primary infection, virus reactivation may lead to nephropathy and graft rejection in renal transplant patients. This study was carried out to access the presence of BKPyV and JCPyV DNA in urine samples collected from renal transplant patients (n = 92) and healthy individuals (n = 88) in Porto Alegre, Rio Grande do Sul. The samples were submitted to a nested PCR. A significantly higher frequency ( P < 0.001) of BKPyV was found in renal transplant patients (65.2%) in comparison to the control group (32.9%). JCPyV was detected equally in both groups. Phylogenetic analysis of both BKPyV and JCPyV amplicons demonstrates the presence of the BKPyV subtypes I and II, whereas for JCPyV, four different groups are found (1, 2, 3, and 4). J. Med. Virol. 87:522–528, 2015 . © 2014 Wiley Periodicals, Inc.