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Potential roles of placental human beta‐defensin‐3 and apolipoprotein B mRNA‐editing enzyme catalytic polypeptide 3G in prevention of intrauterine transmission of hepatitis B virus
Author(s) -
Bai Xiaoxia,
Tian Ting,
Wang Peng,
Yang Xiaofu,
Wang Zhengping,
Dong Minyue
Publication year - 2015
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24072
Subject(s) - hepatitis b virus , virology , biology , placenta , transmission (telecommunications) , trophoblast , immunology , hepatitis b , virus , pregnancy , fetus , genetics , electrical engineering , engineering
Approximately 5% of newborns were infected by hepatitis B virus (HBV) via intrauterine transmission and this is the main reason for high prevalence of HBV in endemic regions. However, the mechanisms by which intrauterine transmission is avoided in most cases remain elusive and placental natural anti‐microbial factors may play a role in the prevention of HBV intrauterine transmission. The expression levels of human β‐defensin‐3 (HBD‐3), apolipoprotein B mRNA‐editing enzyme catalytic polypeptide 3G (A3G) and mannose binding lectin (MBL) were determined in the placenta of 30 HBV‐seronegative pregnant women (controls), 7 HBV‐seropositive pregnant women with infants infected via intrauterine transmission (infected group) and 30 HBV‐seropositive pregnant women with non‐infected infants (non‐infected group). The expression of HBD‐3, A3G, and MBL of placental trophoblast cell line Swan71 was determined after exposed to HBV. There were significant differences in placental HBD‐3 and A3G levels among three groups, but the expression of MBL did not significantly differ. The expressions of HBD‐3 and A3G were higher in non‐infected group than controls and infected group, but not significantly different between infected group and controls. The exposure to HBV increased significantly the expression of HBD‐3, A3G, and MBL by Swan 71. It may be concluded HBV up‐regulates HBD‐3 and A3G expression in vivo and in vitro in placental trophoblast and lack of this up‐regulation is possibly associated with intrauterine transmission of HBV. J. Med. Virol. 87:375–379, 2015 . © 2014 Wiley Periodicals, Inc.