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Direct role of antibody‐secreting B cells in the severity of chronic hepatitis B
Author(s) -
Zgair Ayaid Khadem,
Ghafil Jenan A.,
AlSayidi Razaq H E
Publication year - 2015
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.24067
Subject(s) - antibody , immunology , hepatitis b , medicine , cd8 , alkaline phosphatase , hepatitis b virus , immunoglobulin m , lymphocyte , asymptomatic carrier , hepatitis , immune system , immunoglobulin g , asymptomatic , virology , biology , virus , enzyme , biochemistry
Chronic hepatitis B involves different immune cells. The direct role of antibody‐secreting B cells in the severity of chronic hepatitis B unclear. In this study, the number of plaque forming cells [PFC‐(IgG, IgM, anti‐HBc IgG, and anti‐HBc IgM)], liver function tests (LFT) [alkaline phosphatase (ALP), alanine aminotransferase (ALT), and total serum bilirubin (TSB)], the levels of IL‐10 in sera and in lymphocyte cultures, the number of CD4 + and CD8 + cells were measured in the peripheral blood of patients and in the controls. In addition, the hepatocytotoxic effect of anti‐HBc and anti‐HBe in vitro was studied. The largest number of PFCs was observed in the peripheral blood of patients with chronic hepatitis B. This was concomitant with a decrease in CD4 + /CD8 + ratio versus this ratio in asymptomatic HBV carriers and in healthy volunteers ( P < 0.05). An increase in immunoglobulin (IgG and IgM) levels, anti‐HBc IgG, and anti‐HBc IgM levels and LFTs in peripheral blood of patients with chronic hepatitis B was seen. Anti‐HBc induced hepatocytotoxicity in vitro. The expression of mRNA and protein for IL‐10 production was observed at a significant level in culture of lymphocytes isolated from patients with chronic hepatitis B. In addition, a high level of IL‐10 was found only in the sera of patients with chronic hepatitis B. It is concluded that the antibody‐secreting B cells and the antibodies, which are produced, play an important role in the severity of chronic hepatitis B, which was related negatively with CD4 + /CD8 + ratio and positively with IL‐10 expression. J. Med. Virol. 87:407–416, 2015 . © 2014 Wiley Periodicals, Inc.