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Intranasal immunization with live recombinant Lactococcus lactis combined with heat‐labile toxin B subunit protects chickens from highly pathogenic avian influenza H5N1 virus
Author(s) -
Lei Han,
Peng Xiaojue,
Shu Handing,
Zhao Daxian
Publication year - 2015
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.23983
Subject(s) - lactococcus lactis , virology , immunization , influenza a virus subtype h5n1 , biology , microbiology and biotechnology , virus , nasal administration , influenza a virus , adjuvant , vaccination , immune system , immunology , bacteria , lactic acid , genetics
Development of safe and effective vaccines to prevent highly pathogenic avian influenza H5N1 virus infection is a challenging goal. Lactococcus lactis ( L. lactis ) is an ideal delivery vector for vaccine development, and it has been shown previously that oral immunization of encapsulated secretory L. lactis ‐hemagglutinin (HA) could provide complete protection against homologous H5N1 virus challenge in the mice model. While intranasal immunization is an appealing approach, it is now reported that secretory L. lactis ‐HA combined with mucosal adjuvant heat‐labile toxin B subunit (LTB) could provide protective immunity in the chicken model. As compared to intranasal immunization with L. lactis ‐HA alone, L. lactis ‐HA combined with LTB ( L. lactis ‐HA + LTB) could elicit robust neutralizing antibody responses and mucosal IgA responses, as well as strong cellular immune responses in the vaccinated chickens. Importantly, intranasal immunization with L. lactis ‐HA + LTB could provide 100% protection against H5N1 virus challenge. Taken together, these results suggest that intranasal immunization with L. lactis ‐HA + LTB can be considered as an effective approach for preventing and controlling infection of H5N1 virus in poultry during an avian influenza A/H5N1 pandemic. J. Med. Virol. 87: 39–44, 2015 . © 2014 Wiley Periodicals, Inc.