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Metagenomic sequencing of expressed prostate secretions
Author(s) -
Smelov Vitaly,
Arroyo Mühr L. Sara,
Bzhalava Davit,
Brown Lyndon J.,
Komyakov Boris,
Dillner Joakim
Publication year - 2014
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.23900
Subject(s) - prostate , prostate cancer , metagenomics , biology , secretion , prostatitis , virology , microbiology and biotechnology , gene , cancer , endocrinology , genetics
To investigate which microorganisms may be present in expressed prostate secretions (EPS) metagenomic sequencing (MGS) was applied to prostate secretion samples from five men with prostatitis and five matched control men as well as to combined expressed prostate secretion and urine from six patients with prostate cancer and six matched control men. The prostate secretion samples contained a variety of bacterial sequences, mostly belonging to the Proteobacteria phylum. The combined prostate secretion and urine samples were dominated by abundant presence of the JC polyomavirus, representing >20% of all detected metagenomic sequence reads. There were also other viruses detected, for example, human papillomavirus type 81. All combined prostate secretion and urine samples were also positive for Proteobacteria. In summary, MGS of expressed prostate secretion is informative for detecting a variety of bacteria and viruses, suggesting that a more large‐scale use of MGS of prostate secretions may be useful in medical and epidemiological studies of prostate infections. J. Med. Virol. 86:2042–2048, 2014 . © 2014 Wiley Periodicals, Inc.