Premium
Infection of epithelial cells with dengue virus promotes the expression of proteins favoring the replication of certain viral strains
Author(s) -
MartínezBetancur Viviana,
MarínVilla Marcel,
MartínezGutierrez Marlén
Publication year - 2014
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.23857
Subject(s) - dengue virus , virology , biology , dengue fever , vero cell , viral replication , virus , serotype , cell culture , microbiology and biotechnology , genetics
Dengue virus (DENV) is the causative agent of dengue and severe dengue. To understand better the dengue virus‐host interaction, it is important to determine how the expression of cellular proteins is modified due to infection. Therefore, a comparison of protein expression was conducted in Vero cells infected with two different DENV strains, both serotype 2: DENV‐2/NG (associated with dengue) and DENV‐2/16681 (associated with severe dengue). The viability of the infected cells was determined, and neither strain induced cell death at 48 hr. In addition, the viral genomes and infectious viral particles were quantified, and the genome of the DENV‐2/16681 strain was determined to have a higher replication rate compared with the DENV‐2/NG strain. Finally, the proteins from infected and uninfected cultures were separated using two‐dimensional gel electrophoresis, and the differentially expressed proteins were identified by mass spectrometry. Compared with the uninfected controls, the DENV‐2/NG‐ and DENV‐2/16681‐infected cultures had five and six differentially expressed proteins, respectively. The most important results were observed when the infected cultures were compared to each other (DENV‐2/NG vs. DENV‐2/16681), and 18 differentially expressed proteins were identified. Based on their cellular functions, many of these proteins were linked to the increase in the replication efficiency of DENV. Among the proteins were calreticulin, acetyl coenzyme A, acetyl transferase, and fatty acid‐binding protein. It was concluded that the infection of Vero cells with DENV‐2/NG or DENV‐2/16681 differentially modifies the expression of certain proteins, which can, in turn, facilitate infection. J. Med. Virol. 86:1448–1458, 2014 . © 2013 Wiley Periodicals, Inc.