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Distribution of human papillomavirus genotypes, assessment of HPV 16 and 18 viral load and anal related lesions in HIV positive patients: A cross‐sectional analysis
Author(s) -
Tamalet Catherine,
ObryRoguet Veronique,
Ressiot Emmanuelle,
Bregigeon Sylvie,
Del Grande Jean,
PoizotMartin Isabelle
Publication year - 2014
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.23831
Subject(s) - viral load , genotype , virology , human papillomavirus , hpv infection , medicine , cervical intraepithelial neoplasia , anal cancer , cross sectional study , transmission (telecommunications) , virus , viral disease , cervical cancer , biology , human immunodeficiency virus (hiv) , cancer , pathology , gene , genetics , electrical engineering , engineering
Natural history of anal intraepithelial neoplasia and anal cancer is not fully understood. Factors associated with cytological abnormalities and predictors of progression to high‐grade anal intraepithelial neoplasia still deserve investigation. The aim of this cross‐sectional study was to assess the prevalence of HPV types, the relationship between HPV genotypes, HPV 16/18 viral load and cytological abnormalities in male and female HIV‐infected patients. One hundred and twenty‐two (72.6%) patients were infected with HPV, 75 (61%) had multiple HPV infection, and 94 (77%) had high‐risk HPV infection. The most frequently identified HPV types were HPV 16 (64%), HPV 6 (39%), HPV 18 (31%), HPV 53 (14.7%), HPV 33 (10.6%), HPV 11 (8.2%), HPV 70 (5.7%), and HPV 61 (4.9%). The HPV types which were most frequently found in combination were HPV 6 + 16 (9.8%), 6 + 16 + 18 (8.2%), 16 + 18 (6.6%), 6 + 18 (4.9%), 16 + 33 (3.3%), 16 + 53 (3.3%). Median HPV16 and 18 viral loads were 6.1 log 10 copies/10 6 cells [IQR 5.0–7.3] and 6.1 log 10 copies/10 6 cells [IQR 5.7–6.0], respectively. Male gender ( P = 0.03, OR: 1.2 [1.0–1.4]) and homo/bisexual transmission routes ( P = 0.044, OR: 1.4 [1.0–1.9]) were associated with HPV 16 infection. An HPV 16 viral load cut‐off ≥5.3 log 10 copies/10 6 cells and a CD4+ cell count ≤200/µl were independent factors associated with abnormal cytology. In the absence of national consensus guidelines, a strict regular follow‐up at shorter intervals is recommended for HIV‐infected patients with abnormal cytology, especially low grade squamous intraepithelial lesions, an HPV 16 viral load ≥5.3 log/10 6 cells and a CD4+ cell count ≤200/µl. J. Med. Virol. 86:419–425, 2014 . © 2013 Wiley Periodicals, Inc.