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High prevalence of HCMV and viral load in tumor tissues and peripheral blood of glioblastoma multiforme patients
Author(s) -
dos Santos Claudia Januário,
Stangherlin Lucas Matheus,
Figueiredo Eberval Gadelha,
Corrêa Clemar,
Teixeira Manoel Jacobsen,
da Silva Maria Cristina Carlan
Publication year - 2014
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.23820
Subject(s) - glioblastoma , peripheral blood , virology , viral load , medicine , peripheral , viral disease , virus , biology , pathology , immunology , cancer research
Glioblastoma multiforme is the most prevalent and malignant tumor of the central nervous system. In the last few years, accumulating evidence has suggested an association between human cytomegalovirus (HCMV) infection and glioblastoma multiforme. In this study, tumor tissues and peripheral blood of patients with glioblastoma multiforme were examined for the presence of HCMV DNA. Twenty‐two fresh surgical brain specimens and 20 peripheral blood samples were analyzed by real‐time PCR (qPCR) and hemi‐nested PCR (nPCR) for the presence of pp65 and (glycoprotein B) gB viral genomic regions, respectively. HCMV DNA was detected in the majority of the tumor samples analyzed (95% by qPCR and 91% by nPCR). About half of the patients with tumors positive for HCMV also had detectable viral DNA in their peripheral blood (47% by qPCR and 61% by nPCR). Genome copy numbers were determined and in the majority of the tumor samples cellular DNA outnumbers viral DNA (average of 1 infected cell in 33 cells). The gB genotypes were determined in HCMV‐positive samples and gB2 was the most prevalent genotype in the tumor and blood samples. The results show a high prevalence of HCMV in glioblastoma multiforme samples reinforcing a possible association between HCMV infection and tumor development. J. Med. Virol. 86:1953–1961, 2014 . © 2013 Wiley Periodicals, Inc.