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Prevalence and molecular characterization of G1P[8] human rotaviruses possessing DS‐1‐like VP6, NSP4, and NSP5/6 in Japan
Author(s) -
Kuzuya Mitsutaka,
Fujii Ritsushi,
Hamano Masako,
Kida Koji,
Mizoguchi Yoshinori,
Kanadani Tomohisa,
Nishimura Keiko,
Kishimoto Toshio
Publication year - 2014
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.23746
Subject(s) - reassortment , rotavirus , genotype , biology , virology , genetics , gene , virus , medicine , covid-19 , disease , pathology , infectious disease (medical specialty)
During a survey of human rotaviruses in Okayama Prefecture, Japan in the 2011–2012 rotavirus season (between September 2011 and August 2012), G3P[8] was found to be a predominant genotype overall. However, G1P[8] emerged in the latter half of the season. To clarify the genetic background of the G1P[8] strains, the VP7, VP4, VP6, NSP4, and NSP5/6 genes of the strains were sequenced and genotyped. As a result, it was demonstrated that the strains with two different genotype constellations (G1‐P[8]‐I1‐E1‐H1 and G1‐P[8]‐I2‐E2‐H2) prevailed in the season. The G1P[8] strains possessing the DS‐1‐like VP6, NSP4, and NSP5/6 genes (the DS‐1‐like G1P[8] strains), which should reveal a short electropherotype, were originated from possible intergenogroup reassortment events. The DS‐1‐like G1P[8] strains accounted for 74.1% of all G1P[8] strains and were detected continuously throughout the season but not in the preceding season, indicating the possibility of new introduction and rapid spreading of these strains in the 2011–2012 season. The results suggest that the intergenogroup reassortants, considered generally unstable, can spread rapidly and become relevant. J. Med. Virol. 86:1056–1064, 2014 . © 2013 Wiley Periodicals, Inc.