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Expression changes of cytoskeletal associated proteins in proteomic profiling of neuroblastoma cells infected with different strains of rabies virus
Author(s) -
Zandi Fatemeh,
Eslami Naser,
Torkashvand Fatemeh,
Fayaz Ahmad,
Khalaj Vahid,
Vaziri Behrouz
Publication year - 2013
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.23458
Subject(s) - cytoskeleton , rabies virus , biology , virulence , virus , vimentin , proteomics , rabies , virology , intermediate filament , actin , neurotropic virus , actin cytoskeleton , microbiology and biotechnology , proteome , cell , gene , genetics , immunology , immunohistochemistry
Rabies virus invades the nervous system, induces neuronal dysfunction and causes death of the host. The disruption of the cytoskeletal integrity and synaptic structures of the neurons by rabies virus has been postulated as a possible basis for neuronal dysfunction. In the present study, a two‐dimensional electrophoresis/mass spectrometry proteomics analysis of neuroblastoma cells revealed a significant effect of a virulent strain of rabies virus on the host cytoskeleton related proteins which was quite different from that of an attenuated strain. Vimentin, actin cytoplasmic 1 isoform, profilin I, and Rho‐GDP dissociation inhibitor were host cell cytoskeletal related proteins changed by the virulent strain. The proteomics data indicated that the virulent strain of rabies virus induces significant expression changes in the vimentin and actin cytoskeleton networks of neurons which could be a strong clue for the relation of cytoskeletal integrity distraction and rabies virus pathogenesis. In addition, the expression alteration of other host proteins, particularly some structural and regulatory proteins may have potential roles in rabies virus pathogenesis. J. Med. Virol. 85:336–347, 2013. © 2012 Wiley Periodicals, Inc.