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Relationship between beta‐herpesviruses reactivation and development of complications after autologous peripheral blood stem cell transplantation
Author(s) -
Chapenko Svetlana,
Trociukas Ilze,
Donina Simona,
Chistyakov Maksim,
Sultanova Alina,
Gravelsina Sabine,
Lejniece Sandra,
Murovska Modra
Publication year - 2012
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.23412
Subject(s) - virology , beta (programming language) , transplantation , peripheral blood , stem cell , immunology , medicine , herpesviridae , peripheral , peripheral blood stem cells , biology , viral disease , virus , hematopoietic stem cell transplantation , genetics , computer science , programming language
The relationship between beta‐herpesviruses reactivation and the development of complications after autologous peripheral blood stem cell transplantation was investigated. Viral genomic sequences were detected by the polymerase chain reaction, virus‐specific antibodies by ELISA, and human herpesvirus (HHV)‐6 variants by restriction endonuclease analysis. Virus reactivation, serum levels of tumor necrosis factor (TNF)‐α, interleukin (IL)‐1β, IL‐6, soluble IL‐2 receptor (sIL‐2R), IL‐2, and IL‐4 were compared with clinical features in 44 patients before and after transplantation. Anti‐CMV and anti‐HHV‐6 antibodies were found in 70.5% and 81.8% of patients, respectively. The frequency of plasma viremia was significantly higher in patients after transplantation (41% vs. 11.4%). Reactivation of more than one virus was identified in 55.6% of patients and reactivation of HHV‐7 alone in 44.4%. In cases of concurrent infection, HHV‐7 was reactivated before HHV‐6, and both HHV‐6 and HHV‐7 were reactivated before CMV. There was a significant increase in HHV‐6 load in peripheral blood leukocytes DNA during viremia. In all cases HHV‐6B variant was detected. Complications after transplantation occurred in 27.3% of patients and virus reactivation was detected in all patients with complications. The significant increases in the rate of HHV‐6 and HHV‐7 reactivation and in serum levels of TNF‐α, IL‐1β, and sIL‐2R, as well as aggravated immunosuppression, suggest that both viruses were involved in the complications after autologous peripheral blood stem cell transplantation, via their immunomodulatory activity. The kinetics of reactivation suggests a potential role of HHV‐7 as a co‐factor of HHV‐6 reactivation, and of both HHV‐6 and HHV‐7 as co‐factors of CMV reactivation. J. Med. Virol. 84:1953–1960, 2012. © 2012 Wiley Periodicals, Inc.