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Development of a hepatitis delta virus antibody assay for study of the prevalence of HDV among individuals infected with hepatitis B virus in China
Author(s) -
Shen Liping,
Gu Ying,
Sun Le,
Yang Yingchao,
Wang Feng,
Li Yimin,
Bi Shengli
Publication year - 2012
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.23212
Subject(s) - virology , hepatitis d virus , hbsag , virus , hepatitis b virus , hepatitis d , antigen , antibody , biology , medicine , immunology
Co‐infection with hepatitis delta virus (HDV) and hepatitis B virus (HBV) has been shown to be associated with a more severe form of acute and chronic hepatitis. Cloning and expression of recombinant HDV antigen (rHDAg) in Escherichiacoli are described. Using purified rHDAg, a cost‐effective indirect anti‐HDV enzyme‐linked immunosorbent assay (ELISA) kit was developed. Direct comparison of 15 known HDV‐positive sera and 15 HDV‐negative sera showed concordance agreement between the new assay kit and the Abbott Murex Anti‐Delta (total) kit. In addition, 1,486 hepatitis B surface antigen (HBsAg) positive blood samples collected from various areas of China were tested using this indirect anti‐HDV ELISA. It was found that 1.2% (95% CI: 0.7–1.9%) of the samples were anti‐HDAg positive. It is suggested that the prevalence of HDV and HBV co‐infection in China is relatively low. J. Med. Virol. 84:445–449, 2012. © 2011 Wiley Periodicals, Inc.