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Multicenter assessment of HIV‐1 RNA quantitation in semen in the CREAThE network
Author(s) -
Pasquier Christophe,
Andreutti Corinne,
Bertrand Evelyne,
Bostan Alionka,
Bourlet Thomas,
Molina Irene,
Grossman Zehava,
Halfon Philippe,
LeruezVille Marianne,
LüneborgNielsen Margrethe,
Mar Carmen,
Marcelin AnneGeneviève,
RousselRonserail Catherine,
Schmitt MariePaule,
Tabrizi Sepehr,
Vourliotis Maria,
Bujan Louis
Publication year - 2012
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.23194
Subject(s) - semen , rna , virology , lentivirus , human immunodeficiency virus (hiv) , biology , viral load , virus , andrology , immunology , medicine , viral disease , gene , biochemistry
Detection of HIV‐1 RNA in semen is used commonly to determine the safety of semen processing procedures before assisted reproductive technology (ART). Using two panels of prepared semen samples containing HIV‐1 the performances of protocols from 14 centers have been compared. No false‐positive results were detected but false‐negative results were frequent when the concentration was below 500 HIV‐1 RNA copies/ml of seminal plasma. Frequency of HIV‐1 RNA detection was higher on seminal cells than on seminal plasma. Assays (or protocols) for quantifying HIV‐1 RNA in semen performed less well than standardized blood plasma assays. The HIV load in seminal plasma could be a useful marker of the risk of sexual transmission of the virus. Its use as a marker of global HAART efficiency in the HIV reservoir needs further study. Standardized assays are required for detection and measurement of HIV‐1 RNA in semen samples. J. Med. Virol. 84:183–187, 2012. © 2011 Wiley Periodicals, Inc.