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Phylogenetic analysis of hepatitis B virus genotype F complete genome sequences from Chilean patients with chronic infection
Author(s) -
Venegas Mauricio,
AlvaradoMora Mónica V.,
Villanueva Rodrigo A.,
Rebello Pinho João R.,
Carrilho Flair J.,
Locarnini Stephen,
Yuen Lilly,
Brahm Javier
Publication year - 2011
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.22129
Subject(s) - phylogenetic tree , biology , genbank , virology , hepatitis b virus , genotype , genetics , genome , population , molecular epidemiology , virus , gene , medicine , environmental health
Molecular epidemiological data concerning the hepatitis B virus (HBV) in Chile are not known completely. Since the HBV genotype F is the most prevalent in the country, the goal of this study was to obtain full HBV genome sequences from patients infected chronically in order to determine their subgenotypes and the occurrence of resistance‐associated mutations. Twenty‐one serum samples from antiviral drug‐naive patients with chronic hepatitis B were subjected to full‐length PCR amplification, and both strands of the whole genomes were fully sequenced. Phylogenetic analyses were performed along with reference sequences available from GenBank (n = 290). The sequences were aligned using Clustal X and edited in the SE‐AL software. Bayesian phylogenetic analyses were conducted by Markov Chain Monte Carlo simulations (MCMC) for 10 million generations in order to obtain the substitution tree using BEAST. The sequences were also analyzed for the presence of primary drug resistance mutations using CodonCode Aligner Software. The phylogenetic analyses indicated that all sequences were found to be the HBV subgenotype F1b, clustered into four different groups, suggesting that diverse lineages of this subgenotype may be circulating within this population of Chilean patients. J. Med. Virol. 83:1530–1536, 2011. © 2011 Wiley‐Liss, Inc.