Premium
Mobile and accurate detection system for infection by the 2009 pandemic influenza A (H1N1) virus with a pocket‐warmer reverse‐transcriptase loop‐mediated isothermal amplification
Author(s) -
Hatano Ben,
Goto Megumi,
Fukumoto Hitomi,
Obara Takeyuki,
Maki Takayuki,
Suzuki Go,
Yamamoto Tetsuo,
Hagisawa Kohsuke,
Matsushita Yoshitaro,
Fujii Tatsuya,
Imakiire Toshihiko,
Kikuchi Yuichi,
Takahashi Ryota,
Kanai Mie,
Tamura Kaku,
Izumi Tomoko,
Takahashi Yukihiro,
Iwamoto Yujiro,
Mimura Satoshi,
Mukai Yasuo,
Takita Kazue,
Takeo Hiroki,
Kitamura Ryuichi,
Shimizu Eiichi,
Fukushima Koji,
Hakozaki Yukiya,
Uehata Akimi,
Sakai Masao,
Ohshima Satoshi,
Shirotani Toshiki,
Oba Kunihiro,
Hasegawa Hideki,
Sata Tetsutaro,
Katano Harutaka
Publication year - 2011
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.22031
Subject(s) - reverse transcription loop mediated isothermal amplification , virology , loop mediated isothermal amplification , virus , reverse transcriptase , pandemic , influenza a virus , reverse transcription polymerase chain reaction , medicine , polymerase chain reaction , biology , covid-19 , gene , infectious disease (medical specialty) , messenger rna , dna , biochemistry , genetics , disease , pathology
The 2009 pandemic H1N1 influenza A virus spread quickly worldwide in 2009. Since most of the fatal cases were reported in developing countries, rapid and accurate diagnosis methods that are usable in poorly equipped laboratories are necessary. In this study, a mobile detection system for the 2009 H1N1 influenza A virus was developed using a reverse‐transcriptase loop‐mediated isothermal amplification (RT‐LAMP) kit with a disposable pocket‐warmer as a heating device (designated as pwRT‐LAMP). The pwRT‐LAMP can detect as few as 100 copies of the virus—which is nearly as sensitive as real‐time reverse‐transcription polymerase chain reaction (RT‐PCR)—and does not cross‐react with RNA of seasonal influenza viruses. To evaluate the usefulness of the pwRT‐LAMP system, nasal swab samples were collected from 56 patients with flu‐like symptoms and were tested. Real‐time RT‐PCR confirmed that the 2009 H1N1 influenza A virus was present in 27 of the 56 samples. Of these 27 positive samples, QuickVue Influenza A + B immunochromatography detected the virus in only 11 samples (11/27; 40.7%), whereas the pwRT‐LAMP system detected the virus in 26 of the 56 samples (26/27 of the positive samples; 96.3%). These findings indicate that the mobile pwRT‐LAMP system is an accurate diagnostic system for the 2009 H1N1 influenza A virus, and has great potential utility in diagnosing future influenza pandemics. J. Med. Virol. 83:568–573, 2011. © 2011 Wiley‐Liss, Inc.