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Characteristics of Epstein‐Barr virus isolated from the malignant lymphomas in Korea
Author(s) -
Kim Insun,
Park EuiRyun,
Park SeolHee,
Lin Zhenhua,
Kim YoungSik
Publication year - 2002
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.2193
Subject(s) - lymphoma , epstein–barr virus , virus , tonsil , virology , biology , b cell , palatine tonsil , neoplastic cell , pathology , cell , medicine , immunology , antibody , genetics
Epstein‐Barr virus (EBV) is a common herpes virus linked to a variety of human neoplasms. In this study, the EBV detection was identified with the paraffin‐embedded tissues from 62 non‐Hodgkin's lymphomas, 20 Hodgkin's lymphomas, and 48 non‐neoplastic tonsils, using PCR for EBNA‐1 and EBER‐1 mRNA in situ hybridization for EBER‐1 mRNA. The isolates were analyzed for type 1/2, variants C/D and F/f, and LMP‐1 30 bp deletion. EBV was isolated in 31 of 48 (66%) non‐neoplastic tonsils, 24 of 42 (57%) B cell lymphomas, in 15 of 20 (75%) T cell lymphomas, and 17 of 20 (85%) Hodgkin's lymphomas. These viruses were classified as type 1 for 81% of non‐neoplastic tonsils, 95% of B cell lymphomas, 93% of T cell lymphomas, and 73% of Hodgkin's diseases. Both type 1 and 2 viruses were isolated in one non‐neoplastic tonsil and 3 Hodgkin's diseases. Type C virus was predominant in non‐neoplastic tonsils (77%) and B cell lymphomas (75%), while type D virus was common in T cell lymphomas (71%) and Hodgkin's diseases (73%) ( P < 0.05). Majority of the viruses detected in non‐neoplastic tonsils (93%) and malignant lymphomas (91%) were “F” prototype. LMP‐1 30 bp deletion was found in high frequency in both non‐neoplastic tonsils (92%) and malignant lymphomas (86%). In conclusion, most of EBV found in Korea was type 1, and “DF” genotype was more frequent in T cell lymphomas and Hodgkin's diseases than in non‐neoplastic tonsils and B cell lymphomas. LMP‐1 30 bp deletion did not seem to be associated with malignant lymphomas. J. Med. Virol. 67:59–66, 2002. © 2002 Wiley‐Liss, Inc.