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Low prevalence of HPV in Brazilian children with retinoblastoma
Author(s) -
Antoneli Célia B.G.,
Ribeiro Karina B.,
Sredni Simone T.,
Arias Victor E.A.,
Andreoli Maria A.,
de Camargo Beatriz,
Sobrinho João S.,
Prado José C.M.,
Soares Fernando A.,
Villa Luisa L.
Publication year - 2011
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.21925
Subject(s) - retinoblastoma , enucleation , polymerase chain reaction , medicine , nested polymerase chain reaction , pathology , biology , virology , gene , genetics , surgery
Retinoblastoma is the most frequent intra‐ocular malignant tumor of the childhood, occurring in 1 of 18,000–30,000 live births. Little is known about the causes of sporadic retinoblastoma and only a few authors have investigated the etiologic role of human papillomavirus (HPV), with controversial results. Formalin‐fixed, paraffin‐embedded tissue blocks containing retinoblastoma were retrieved from the archives of the Department of Pathology at Hospital A C Camargo, São Paulo, Brazil. All patients were treated with enucleation (21 children had both eyes enucleated). Retinoblastoma and, when possible, normal retina of each specimen, were micro‐dissected under direct light microscopic visualization by using a PixCell II Laser Capture Micro‐dissection System. The DNA quality was evaluated by polymerase chain reaction (PCR) amplification of 110 base pairs fragment of the human β‐globin gene using primers PCO3+/PCO4+. All globin positive specimens were analyzed by PCR for the presence of HPV DNA using consensus primers GP5+/GP6+. A total of 154 specimens were evaluated. Forty‐four patients also had normal retinal specimens available for analysis of DNA HPV. The DNA HPV prevalence among all tumor specimens was 4.6% (95% CI 2.0; 8.8) (7 positive specimens/153 adequate specimens). Among normal retinal specimens, the DNA HPV prevalence was 9.1% (95% CI 2.9; 20.5) (4 positive specimens/44 specimens). There was no statistically significant difference between these rates ( P = 0.318). Excluding any experimental failure, our results indicate a low prevalence of HPV DNA in retinoblastomas. We were therefore unable to conclude about the association between these oncogenic viruses and this rare pediatric neoplasm. J. Med. Virol. 83:115–118, 2011. © 2010 Wiley‐Liss, Inc.