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Cellular tropisms and co‐receptor usage of HIV‐1 isolates from vertically infected children with neurological abnormalities and rapid disease progression
Author(s) -
McCarthy Micheline,
He Jun,
Auger Denise,
Geffin Rebeca,
Woodson Cristina,
Hutto Cecelia,
Wood Charles,
Scott Gwendolyn
Publication year - 2002
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.2185
Subject(s) - biology , virology , microglia , chemokine receptor , astrocyte , immunology , phenotype , receptor , cxcr4 , chemokine receptor ccr5 , chemokine , central nervous system , genetics , inflammation , gene , neuroscience
The longitudinal evolution of HIV‐1 phenotypes was studied in a cohort of six vertically infected children with early onset and rapid progression of clinical disease. Among 30 viral isolates obtained from peripheral blood, tropisms for both human blood‐derived cells (macrophages, T‐lymphocytes), and for human neural (brain‐derived) cells (microglia, astrocytes) were determined, as was chemokine co‐receptor usage. All children harbored from birth macrophage‐tropic isolates using the CCR5 co‐receptor. Two children later developed T‐cell tropic isolates with CXCR4 and CCR3 usage. While all six patients developed neurological abnormalities, only three produced neural cell tropic isolates, which used CCR5. However, early and persistent finding of both astrocyte‐ and microglia‐tropic isolates in one patient did associate with the most rapid progression to brain atrophy among the six patients. Viral phenotypic properties determined in cell culture did not specifically predict clinical features or course, and the development of AIDS did not coincide with, or depend on, the appearance T‐tropic, syncytia‐inducing viruses. J. Med. Virol. 67:1–8, 2002. © 2002 Wiley‐Liss, Inc.