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Quantitative and qualitative assay of rubella IgA antibody in breast milk
Author(s) -
Hayakawa Yuko,
Zhou Yumei,
Mizuguchi Masashi,
Frey Teryl K.,
Ushijima Hiroshi
Publication year - 2010
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.21838
Subject(s) - colostrum , rubella virus , rubella , breast milk , titer , virology , antibody , hemagglutination assay , virus , immunoglobulin a , medicine , immunology , biology , vaccination , immunoglobulin g , measles , biochemistry
Breast milk contains immunological factors, such as IgA antibody, which help to prevent infectious diseases. A total of 197 paired samples of colostrum and breast milk was collected from postpartum mothers in Gunma City, Japan, and examined for anti‐rubella IgA antibody by enzyme‐linked immunosorbent assay (ELISA) and Western blotting (WB). The anti‐rubella virus IgA ranged from 0.5 to 78.5 U/ml with a mean of 6.05 U/ml and a median of 3.6 U/ml in colostrum, and from 0.5 to 32.7 U/ml with a mean of 2.74 U/ml and a median of 2 U/ml in milk. The differences between the means of titers of total IgA and anti‐rubella virus IgA in colostrum and in milk were significant statistically. The levels of anti‐rubella virus IgA in both colostrum and breast milk correlated positively with the anti‐rubella virus hemagglutination inhibition (HI) titers in the sera of mother, indicating that the levels of these different classes of antibodies correlated. Based on WB, anti‐rubella virus IgA in both colostrum and breast milk reacted with the rubella viral protein E1 and C, but not with the E2 protein. J. Med. Virol. 82:1475–1479, 2010. © 2010 Wiley‐Liss, Inc.