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Genotyping and characterization of the geographical distribution of tick‐borne encephalitis virus variants with a set of molecular probes
Author(s) -
Demina T.V.,
Dzhioev Yu.P.,
Verkhozina M.M.,
Kozlova I.V.,
Tkachev S.E.,
Plyusnin A.,
Doroshchenko E.K.,
Lisak O.V.,
Zlobin V.I.
Publication year - 2010
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.21765
Subject(s) - genotype , genotyping , biology , tick borne encephalitis virus , virology , virus , encephalitis , nucleic acid , genetics , gene
A panel of deoxyoligonucleotide probes for studying the genetic variability and genotyping of Tick‐borne encephalitis virus (TBEV) strains by molecular hybridization of nucleic acids (MHNA) was created. This panel allows to estimate the genetic structure of individual TBEV strains, as the targets for probes are both variable and genotype (subtype)‐specific sequences of all TBEV genes. With the help of this panel using the method of molecular hybridization of nucleic acids 268 archived TBEV isolates were investigated and the distribution of its genotypes and subgenotypes of genotype 3 was made more precise in the territory of Eurasia. The conclusion made earlier has confirmed that five genotypes of TBEV co‐circulate in Eastern Siberia. It is generally recognized that the Far Eastern (TBE‐FE), European (TBE‐Eu), and Siberian (TBE‐Sib) genotypes are widespread and epidemiologically important. The fourth genotype is presented by only one isolate, TBE 178‐79 , originated from Irkutsk region, Russia. The fifth genotype includes 10 isolates, 1 of them, TBE 886‐84 , was found earlier and recognized as unique [Zlobin et al. (2001b): Vopr Virusol 1:12–16 (Russian)]. J. Med. Virol. 82:?–?, 2010. © 2010 Wiley‐Liss, Inc.