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High prevalence of species D human adenoviruses in fecal specimens from Urban Kenyan children with diarrhea
Author(s) -
Magwalivha Mpho,
Wolfaardt Marianne,
Kiulia Nicholas M.,
van Zyl Walda B.,
Mwenda Jason M.,
Taylor Maureen B.
Publication year - 2010
Publication title -
journal of medical virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 121
eISSN - 1096-9071
pISSN - 0146-6615
DOI - 10.1002/jmv.21673
Subject(s) - diarrhea , biology , genotype , feces , serotype , diarrheal disease , virology , veterinary medicine , medicine , microbiology and biotechnology , biochemistry , gene
Human adenoviruses (HAdVs) cause a wide range of clinical syndromes and are classified in seven species, A–G, comprising 52 serotypes. HAdV‐A31, ‐F40, and ‐F41 have been associated with diarrhea in infants and young children. In developing countries gastroenteritis is a major cause of morbidity and mortality in children and, in comparison to rotaviruses, there are no data on the HAdVs associated with diarrhea in pediatric patients in Kenya. This study investigates the prevalence and genotypes of HAdVs in 278 stool specimens (211 diarrheal; 67 non‐diarrheal) from children ≤14 years of age in urban and rural areas in Kenya. Stool specimens were screened for HAdVs using a nested polymerase chain reaction and the HAdVs genotyped by sequence analysis of a conserved hexon gene fragment. HAdVs were detected in 104/278 (37.4%) of the stool specimens: 35/43 (81.4%) of diarrheal and 10/61 (16.4%) of non‐diarrheal stool specimens from children in an urban hospice; 25/94 (26.6%) of diarrheal specimens from urban children and 34/80 (42.5%) of diarrheal specimens from children in a rural area. Species D HAdVs were identified as the most prevalent HAdV species in diarrheal stool specimens from urban children comprising 18/37 (48.6%) of the strains identified. In contrast HAdV species F predominated in pediatric diarrheal specimens from the rural area, being identified in 7/16 (43.8%) of the characterized strains. This study provides valuable new data on the prevalence and distribution of HAdV genotypes in diarrheal stool specimens in Kenya and Africa, and highlights the necessity for further investigations. J. Med. Virol. 82:77–84, 2010. © 2009 Wiley‐Liss, Inc.